Daffy Duck appeared in the 1988 film Who Framed Roger Rabbit alongside Donald Duck in a piano duel scene.

The Wikipedia entry for Daffy Duck describes his appearance in Who Framed Roger Rabbit (1988), where he was one of many Looney Tunes characters allowed by Warner Bros. to appear. In the film, Daffy shares a scene with his Disney counterpart Donald Duck while performing in a piano duel. He was animated by Dave Spafford for this sequence. This crossover is notable because it brought together two of animation's most famous ducks from rival studios in a single frame, a moment that would have been unthinkable during the height of the Disney-Warner Bros. rivalry.